Mycobacteria tuberculosis epidemiology and resistance detection by using NGS
Next Generation Sequencing (NGS) allows for rapid analysis of nearly complete genomes of clinical Mycobacterium tuberculosis complex (MTBC) isolates. NGS based whole genome sequencing (WGS) analysis goes far beyond conventional molecular tests e.g. for drug susceptibility testing by detection of variants in virtually all target genes involved in resistance development (resistome analysis). Simultaneously, it allows for high-resolution strain comparison e.g. for outbreak analysis or even longitudinal genome based molecular epidemiological studies. The application of WGS for diagnostics and strains comparison has become realistic due to the development on so-called bench top NGS systems that can be integrated into a normal laboratory workflow, and allow for WGS of clinical isolates few days and at low costs.